{"id":67628,"date":"2017-10-27T10:25:52","date_gmt":"2017-10-27T08:25:52","guid":{"rendered":"https:\/\/haberler.dk\/tr\/?p=67628"},"modified":"2017-10-27T10:25:52","modified_gmt":"2017-10-27T08:25:52","slug":"dnada-degisiklik-yapmayi-kolaylastiran-enzim-kesfedildi","status":"publish","type":"post","link":"https:\/\/www.nordicturk.com\/arsiv\/20122025\/dnada-degisiklik-yapmayi-kolaylastiran-enzim-kesfedildi\/67628\/","title":{"rendered":"DNA&#8217;da de\u011fi\u015fiklik yapmay\u0131 kolayla\u015ft\u0131ran enzim ke\u015ffedildi"},"content":{"rendered":"<p>Bilim adamlar\u0131, DNA&#8217;daki genomlarda do\u011frudan ve kal\u0131c\u0131 de\u011fi\u015fiklik yapmay\u0131 sa\u011flayan yeni bir t\u00fcr enzim buldu.<\/p>\n<p>Howard Hughes T\u0131p Enstit\u00fcs\u00fcnden (HHMI) ara\u015ft\u0131rmac\u0131 David Liu ve ekibi, yapt\u0131klar\u0131 ara\u015ft\u0131rmada, DNA&#8217;da bulunan A*T (Adenin*Timin) baz\u0131n\u0131 G*C (Guanin*Sitozin) baz\u0131na do\u011frudan d\u00f6n\u00fc\u015ft\u00fcrmeyi kolayla\u015ft\u0131ran bir enzim ke\u015ffetti.<\/p>\n<p>\u0130nsanlarda g\u00f6r\u00fclen hastal\u0131klarla ili\u015fkili 32 bin mutasyonun neredeyse yar\u0131s\u0131na yol a\u00e7an G*C baz\u0131n\u0131n A*T baz\u0131na d\u00f6n\u00fc\u015f\u00fcm\u00fcn\u00fc tersine \u00e7evirmek isteyen bilim adamlar\u0131, daha \u00f6nceki deneylerinde C*G baz\u0131n\u0131, T*A baz\u0131na d\u00f6n\u00fc\u015ft\u00fcrmeyi ba\u015farm\u0131\u015ft\u0131.<\/p>\n<p>Adenini h\u00fccrelerin &#8220;guanin&#8221; gibi alg\u0131lad\u0131\u011f\u0131 inosin molek\u00fcl\u00fcne \u00e7eviren TadA enzimi ve bunun mutasyonlar\u0131n\u0131 inceleyen bilim adamlar\u0131, yapt\u0131klar\u0131 deneylerde bakterilerde olu\u015fan mutasyonlar\u0131n bir k\u0131sm\u0131n\u0131 ortadan kald\u0131rmay\u0131 ba\u015fard\u0131.<\/p>\n<p>Biyolog Liu ve ekibi, daha sonra TadA enzimini, gen makas\u0131 olarak adland\u0131r\u0131lan ve genleri DNA \u00fczerinde keserek k\u0131rpmaya ve kopyalamaya izin veren CRISPR (d\u00fczenli aral\u0131klarla b\u00f6l\u00fcnm\u00fc\u015f palindromik tekrar k\u00fcmeleri) Cas9 enzimine ba\u011flad\u0131.<\/p>\n<p>Bilim adamlar\u0131, deneyler s\u0131ras\u0131nda ABE7.10 enzimini kullanarak hem insan hem de bakteri genlerinde y\u00fczde 50&#8217;nin \u00fczerinde verimlilik ve \u00e7ok az istenmeyen mutasyonla A*T baz \u00e7iftini G*C \u00e7iftine d\u00f6n\u00fc\u015ft\u00fcrmeyi ba\u015fard\u0131.<\/p>\n<p>Liu, ara\u015ft\u0131rman\u0131n sonu\u00e7lar\u0131n\u0131n insanlar\u0131n tedavisinde kullan\u0131lmas\u0131 i\u00e7in hen\u00fcz erken oldu\u011funu belirtti.<\/p>\n<p>Gen m\u00fchendisli\u011fi alan\u0131nda \u00e7\u0131\u011f\u0131r a\u00e7mas\u0131 beklenen ke\u015ffin, gelecekte insanlarda hastal\u0131klara neden olan mutasyonlar\u0131 d\u00fczeltecek DNA operasyonlar\u0131nda kullan\u0131lmas\u0131 \u00f6ng\u00f6r\u00fcl\u00fcyor.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Bilim adamlar\u0131, DNA&#8217;daki genomlarda do\u011frudan ve kal\u0131c\u0131 de\u011fi\u015fiklik yapmay\u0131 sa\u011flayan yeni bir t\u00fcr enzim buldu.<\/p>\n","protected":false},"author":3,"featured_media":9387,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jnews-multi-image_gallery":[],"jnews_single_post":[],"jnews_primary_category":[],"footnotes":""},"categories":[22],"tags":[],"coauthors":[],"class_list":["post-67628","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bilim"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>DNA&#039;da de\u011fi\u015fiklik yapmay\u0131 kolayla\u015ft\u0131ran enzim ke\u015ffedildi<\/title>\n<meta name=\"description\" content=\"Bilim adamlar\u0131, DNA&#039;daki genomlarda do\u011frudan ve kal\u0131c\u0131 de\u011fi\u015fiklik yapmay\u0131 sa\u011flayan yeni bir t\u00fcr 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